Fig1: Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3
Fig2: ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Product Name | Filamin A [SA30-08] |
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Antibody Type | Primary Antibodies |
Product description |
|
Immunogen | recombinant protein |
Clonality | Monoclonal |
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Isotype | IgG |
Host Species | Recombinant rabbit |
Tested Applications | |
WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:400 FC:1:10-1:100 |
|
Species Reactivity | |
Concentration | 1 mg/mL. |
Alternative Names | ABP 280 antibody
ABP-280 antibody
Actin-binding protein 280 antibody
Alpha filamin antibody
Alpha-filamin antibody
APBX antibody
CSBS antibody
CVD1 antibody
Endothelial actin binding protein antibody
Endothelial actin-binding protein antibody
Filamin 1 antibody
Filamin A alpha antibody
Filamin A antibody
Filamin-1 antibody
Filamin-A antibody
FLN antibody
FLN-A antibody
FLN1 antibody
FLNA antibody
FLNA_HUMAN antibody
FMD antibody
MNS antibody
NHBP antibody
Non muscle filamin antibody
Non-muscle filamin antibody
OPD antibody
OPD1 antibody
OPD2 antibody
XLVD antibody
XMVD antibody
|
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Molecular Weight(MW) | 281 kDa |
Cellular Localization | Cytoplasm |
SwissProt ID | P21333 Q8BTM8 4213 |
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Fig1: Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3
Fig2: ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Positive Control | HUVEC, MCF-7, Hela, Ags, Jurkat, NIH/3T3, mouse uterus tissue, human uterus tissue. |
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Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:400 FC:1:10-1:100 |
Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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